PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells.

PUS7-mediated pseudouridylation of 7SK enhances human somatic cell reprogramming efficiency and chromatin accessibility.

• Why it matters: Understanding the molecular regulation of reprogramming is crucial for advancing regenerative medicine, yet key mechanisms remain unclear.
• What they did: The study identified PUS7 as an RNA modification checkpoint, showing that its loss impairs reprogramming while overexpression accelerates it; it modifies 7SK at U250, promoting interaction with the BAF complex.
• The result: This modification influences chromatin remodeling, and the PSKM reprogramming framework produces high-quality iPS cells with faithful imprinting, robust lineage potential, and efficient differentiation.