Temporal transcriptomics identifies transregulation by multiple lncRNAs in human iPSCs.

Inducible lncRNAs in human iPSCs reveal trans-regulatory effects on specific gene sets, with six lncRNAs identified as regulators of distinct transcript expression.

• Why it matters: Understanding how lncRNAs influence gene and epigenetic regulation over time is limited, especially regarding their trans-regulatory mechanisms, which are crucial for deciphering gene expression control in pluripotent stem cells.
• What they did: The study engineered 17 doxycycline-inducible lncRNA transgenes in human iPSCs and generated high-density temporal RNA-seq and ATAC-seq profiles, identifying six lncRNAs with trans-regulatory activity.
• The result: Target genes of these lncRNAs include 53 mRNAs and 10 lncRNAs, with LINC00847’s targets enriched on Chromosome 19, highlighting specific trans-regulatory networks and providing a resource to distinguish artifacts from genuine regulatory effects.