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Single-Nucleus RNA Sequencing Reveals WRKY12a Regulating Secondary Cell Wall Formation During Xylem Differentiation in Rubber Tree.
Plant, Cell & Environment · · Journal Article
Pan, Xu + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
HbWRKY12a is enriched in fibre-vessel cells of Hevea brasiliensis and directly activates HbMYB1R1c to regulate secondary cell wall formation during xylem differentiation.
- Why it matters: Secondary xylem differentiation shapes wood structure and function, but its regulatory mechanisms in Hevea brasiliensis remain poorly resolved at cell-type resolution.
- What they did: The authors built a cambium-to-mature xylem snRNA-seq atlas, used pseudotime and gene regulatory network analysis to identify HbWRKY12a, and confirmed direct binding/activation of HbMYB1R1c with molecular assays.
- The result: Overexpressing HbWRKY12a or HbMYB1R1c in Arabidopsis significantly reduced stem diameter and plant height and markedly decreased SCW thickness in fibre, vessels, and pith cells.
The findingWhy it mattersWhat they didThe result
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