The abstract is read at the publisher; the summary is JClub's.
Yeast nuclei carrying intact synthetic megabase-scale human DNA were delivered into mouse early embryos with 100% microinjection delivery efficiency while maintaining a naive state.
- Why it matters: Cross-species transfer of megabase-scale synthetic DNA into mammalian cells is difficult, limiting synthetic genomics studies of how new epigenetic states form and regulate transcription over time.
- What they did: The NICE protocol isolates yeast nuclei containing intact synthetic megabase-scale DNA with preserved chromatin structure, then microinjects high-concentration, high-purity nuclei into mouse early embryos. Isolated nuclei can be stored at -80 °C for >6 months, and the full workflow including pulsed-field gel electrophoresis verification takes ~5 d.
- The result: The delivered nuclei enable time-resolved study of de novo epigenetic modification establishment and its influence on transcriptional regulation in mammalian embryos, using a platform that is efficient and reliable.
The findingWhy it mattersWhat they didThe result
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