The abstract is read at the publisher; the summary is JClub's.
FEBID tips made with a benchtop SEM enable high-resolution, long-term stable HS-AFM imaging of annexin V and plasmid DNA, with eight tips fabricated in 1–2 h.
- Why it matters: HS-AFM image resolution depends strongly on tip sharpness, but many labs avoid FEBID because they lack SEM access or face technical barriers to making reproducible AFM tips.
- What they did: The team built a cantilever and sample holder to reduce drift and vibrations during SEM operation, then varied beam defocus, deposition time, acceleration voltage, and beam current to optimize FEBID tip shape.
- The result: Using the optimized workflow, they achieved high-resolution HS-AFM movies and sustained imaging stability for annexin V and plasmid DNA, supporting reproducible AFM data acquisition.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.