Proteomic Evaluation of the Progression from Acute Kidney Injury to Chronic Kidney Disease

Quantitative DIA-MS proteomics in mouse AKI (ischemia-reperfusion) and CKD (unilateral ureteral obstruction) identified 5,985 protein groups and 95 candidate progression markers.

• Why it matters: The molecular steps linking acute kidney injury to chronic fibrosis are not fully defined, limiting biomarker discovery and targeted therapies for this transition.
• What they did: The study profiled mouse kidneys using data-independent acquisition mass spectrometry, finding a 1,475-protein (46%) shared injury response and tracking peroxisomal, SASP-like, and fibrosis-related changes.
• The result: Peroxisomal proteins, including Acox1 and Ehhadh, were consistently down-regulated in both AKI and CKD with no peroxisomal up-regulation, and seven co-expression modules plus Brat1 and Col3a1 supported progression marker discovery.

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