Engineered Miniature ParTasR Enables Efficient PAMless Genome Editing

eTasR, an engineered ParTasR variant fused to TREX2, boosts PAMless genome editing in mammalian cells from 4.29% to 48.94%, averaging a 26.52-fold increase.

• Why it matters: Low editing efficiency of TIGR-Tas in mammalian cells limits PAMless genome engineering, including potential therapeutic use.
• What they did: Using structure-guided rational design of ParTasR plus TREX2 fusion, the authors compared editing efficiency to SpRY and tested single-AAV delivery for in vivo Hpd disruption.
• The result: Single-AAV delivery of eTasR enabled efficient Hpd disruption in vivo and produced robust phenotypic rescue, supporting a compact PAMless platform for genome editing.

AI summary of the abstract · may contain errors · not medical advice · read the paper.