The abstract is read at the publisher; the summary is JClub's.
RAMP-Seq is a single-tube multiplex nanopore workflow that co-amplifies full-length 16S (~1,500 bp) and eight AMR determinants (~600–700 bp) for integrated taxonomic and resistance profiling.
- Why it matters: AMR surveillance needs one-sample workflows that identify bacteria and detect resistance genes without splitting steps into separate reactions or library preparations.
- What they did: The method uses a single multiplex PCR with a non-equimolar primer pool, then sequences the mixed-length amplicon pool on nanopores; it reports 8.2 {+/-} 0.6 h from extracted DNA to first taxonomically classified reads.
- The result: In an eight-member mock community it recovered all expected taxa with Pearson’s r = 0.879, and in eleven multidrug-resistant isolates it identified all seven panel targets present, while spiked Klebsiella pneumoniae was recovered down to 102 CFU/mL after host DNA depletion.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.