The abstract is read at the publisher; the summary is JClub's.
Rejuvenation rankings from CRISPRi knockdowns are not stable across aging cohorts, with fibroblast donor versus GTEx fibroblast rankings showing no correlation (ρ = -0.05).
- Why it matters: Ranking knockdowns by reversal of an aging expression signature is widely used, but it is unclear whether the resulting rejuvenation targets persist across different human aging cohorts.
- What they did: TRACE scored 18,746 CRISPRi knockdown profiles from five screens in four human cell lines, comparing rankings derived from 111 donor fibroblasts versus GTEx fibroblasts.
- The result: Raw scores tracked cell growth programs within each signature—strongly linking proliferation to fibroblast signatures and weakly linking stress to GTEx signatures—while cohort-specific ranking agreement was limited (ρ = -0.05; exploratory ρ = -0.55).
The findingWhy it mattersWhat they didThe result
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