High +Gz Exposure Alters Urinary Extracellular Vesicle miRNAs in Humans

Six urinary extracellular vesicle–encapsulated miRNAs increased significantly after acute high +Gz exposure in 21 healthy young men, with three transient and three sustained elevations.

• Why it matters: The molecular signals linked to acute high +Gz cardiovascular, autonomic, and endocrine responses in humans are poorly defined, limiting understanding of systemic stress biology.
• What they did: Urine was collected before, immediately after, and the morning after centrifuge training; uEV miRNAs were profiled by RNA sequencing and Gene Ontology, then 16 ischemia/hypoxia/blood pressure/mechanical-stress–related miRNAs were quantified by qPCR.
• The result: miR-20b-5p, miR-125b-5p, and miR-130a-3p rose immediately and returned to baseline, while miR-335-5p, miR-17-5p, and miR-92a-3p stayed elevated; catecholamines and salivary cortisol and heart rate increased, paralleling distinct temporal patterns.

AI summary of the abstract · may contain errors · not medical advice · read the paper.