An interplay of multilayered regulatory mechanisms underlies the RNA solubility phase transition during vertebrate oocyte-to-embryo transition

Fractionation RNA-seq in zebrafish during oocyte-to-embryo transition shows many maternal transcripts switch between soluble and insoluble states, with ribosome-dependent and ribosome-independent drivers.

• Why it matters: Global RNA–protein interaction dynamics during OET are poorly defined, despite transcriptional silence and the need for spatiotemporal control of maternal transcripts.
• What they did: The study profiled RNA insolubility via fractionation RNA-seq across the zebrafish OET, then built a machine-learning framework to link cis- and trans-regulatory features to insolubility, validating with poly(A) tail length and m6A dynamics.
• The result: Poly(A) tail length tracks both ribosome-dependent and ribosome-independent RNA phase transitions, while fractionation m6A-pulldown RNA-seq shows a gradually established correlation between m6A modifications and RNA insolubility during the OET.

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