Global mapping of VEGF-A driven changes in the RNA bound phospho-proteome reveals a molecular switch to coordinate angiogenesis in endothelial cells

Phf6 phosphorylation at serine 199 in mouse cardiac endothelial cells is consistently increased after VEGF-A stimulation and acts as a molecular switch for angiogenesis-linked RNA programs.

• Why it matters: Angiogenesis requires coordinated post-transcriptional control, but how VEGF-A reshapes RNA-bound protein phosphorylation to reprogram endothelial RNA fate is unclear.
• What they did: The study performed phospho-proteomic profiling of the mRNA bound proteome in mouse cardiac ECs under acute and prolonged VEGF-A stimulation, then tested whether Phf6 serine 199 phosphorylation was sufficient and required.
• The result: Phf6 serine 199 phosphorylation downstream of VEGF-A differentially coordinates reduced anti-angiogenic extracellular matrix RNAs and increased pro-angiogenesis RNAs, partly by altering RNA stability, to promote angiogenesis.

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