The abstract is read at the publisher; the summary is JClub's.
Viral-derived HUH endonuclease tags enable efficient, site-specific conjugation of recombinant IL-1β–HUH to unmodified ssDNA within minutes, supporting tunable protein-decorated DNA origami.
- Why it matters: Protein functionalization of DNA origami is a bottleneck that limits spatial studies of receptor signaling to specialized labs. A simpler, cheaper method using standard equipment and unmodified DNA is needed.
- What they did: The authors used small (~10 kDa) HUH domains that form a covalent phosphotyrosine bond with short unmodified ssDNA, then coupled IL-1β–HUH conjugates to a DNA origami pegboard with tunable stoichiometry.
- The result: IL-1β–functionalized origami triggered ligand-dependent signaling in living cells and enabled visualization of downstream signaling molecule recruitment, indicating HUH-tag conjugation preserves protein functionality.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.