The abstract is read at the publisher; the summary is JClub's.
N-terminally truncating legume prenyltransferases enables high bakuchiol production in Yarrowia lipolytica, reaching 6.03 g/L in a 7.5-L fed-batch bioreactor.
- Why it matters: Enzymes that support bakuchiol biosynthesis are not fully mapped, restricting the toolkit for microbial pathway engineering and limiting achievable titers.
- What they did: A chromosome-level Psoralea corylifolia genome (471.76 Mb; contig N50 40.33 Mb; 11 pseudochromosomes) guided cross-species screening, yielding four prenyltransferases; truncating PcPT6 (PcPT07) by 40 amino acids increased production by 53%.
- The result: Rebuilt bakuchiol biosynthesis in Y. lipolytica with reinforced p-coumaric acid supply and coordinated mevalonate pathway reinforcement plus increased t40PcPT6 dosage, achieving 108.29 mg/L in shake flasks and 6.03 g/L in fed-batch.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.