The abstract is read at the publisher; the summary is JClub's.
Clonal lineage recording in mouse embryos shows paraxial mesoderm origins diverge after primitive streak formation yet converge on extensively overlapping developmental outputs, contradicting a simple branching lineage tree.
- Why it matters: Fate-mapping and single-cell trajectories have implied progressive fate bias and branching segregation, but a clonal, spatially resolved map of how mesoderm diversity emerges during gastrulation is still missing.
- What they did: Inducible CRISPR-based lineage recording was combined with single-cell RNA sequencing, barcoding mouse embryos at primitive streak onset and analyzing clonal relationships at early organogenesis using sampling-aware statistical models.
- The result: Endothelial cells arise from multiple mesoderm origins with transcriptional differences tied to anatomical position and clonal lineage, and clonal sharing links extra-embryonic mesoderm to cardiac lineages and a shared hindlimb-allantois progenitor.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.