The abstract is read at the publisher; the summary is JClub's.
A repertoire of 29 nanobodies against the bacterial amino acid transporter BasC shows strong intracellular-face bias, with only Nb58 and Nb78 binding extracellularly.
- Why it matters: Systematically mapping large nanobody collections to transporter binding sites is experimentally demanding, limiting how well researchers can prioritize candidates and interpret structural data.
- What they did: The team combined transport assays in proteoliposomes, experimental structural data, and AlphaFold3 modeling; they used sidedness from influx versus efflux inhibition and validated with a 6.1 [A] crystallographic map.
- The result: Ten AlphaFold3 interaction models targeted the large intracellular cavity, revealing recurrent epitope clusters, and predicted modes for Nb53, Nb71, and Nb74 matched existing structures, enabling virtual screening and compatible combinations.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.