Synchrotron diffraction reveals distinct collagen-associated distributions of EGFR-targeted antibody and affibody in pancreatic tumors

Cetuximab IRDye700DX shows consistently negative spatial correlations with collagen-associated diffraction in orthotopic pancreatic tumors in 12 athymic nude mice, across 100–400 µm bins.

• Why it matters: Tumor extracellular matrix structure may shape how targeted agents distribute, yet it is unclear whether different molecular formats targeting the same receptor relate similarly to collagen-associated tissue architecture.
• What they did: Cetuximab IRDye700DX and the EGFR-targeted affibody ABY029 were coadministered to 12 mice bearing orthotopic AsPC1 (n=8) or PANC1 (n=4) tumors, then imaged at 3 or 24 h and registered to synchrotron XRD collagen-associated intensity.
• The result: Cetuximab correlations were negative in all mice at every binning scale (all adjusted P=0.0039), and cohort-average correlations were more negative than ABY029 at all four scales (paired adjusted P=0.0137–0.0381).

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