The abstract is read at the publisher; the summary is JClub's.
Single-nucleus long-read RNA sequencing in acutely injured C57BL/6J mouse kidneys shows ischemia-reperfusion injury shifts isoform composition in proximal tubule cells, including oxidative phosphorylation-associated genes.
- Why it matters: Short-read single-cell/nucleus RNA-seq can’t reliably identify isoforms, limiting characterization of protein-coding and non-protein-coding isoforms during kidney injury.
- What they did: They generated single-cell and single-nucleus long-read RNA-seq from kidneys of three healthy adult male C57BL/6J mice and performed bilateral ischemia-reperfusion injury in three more, then compared isoform usage.
- The result: Across 1.3 billion reads (median 757–884 bases), single-nucleus libraries detected more distinct isoforms and revealed injury-linked isoform shifts, including prominent changes for Fut9 and Prrc2b.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.