The abstract is read at the publisher; the summary is JClub's.
Combined-FISH quantifies sul1 copy number in taxonomically identified single bacterial cells, detecting 1–8 copies in Escherichia coli and 1–4 in Acinetobacter defluvii per cell.
- Why it matters: In complex microbial environments, linking antibiotic resistance genes to their in situ hosts is difficult because ARGs often reside on mobile elements.
- What they did: The authors combined direct-geneFISH for sul1 detection with CARD-FISH rRNA labeling, using a probe set covering all non-redundant sul1 sequences within 5% mismatch, and validated it in plasmid-harboring model organisms.
- The result: After plasmid-mediated transfer from Escherichia coli to Pseudomonas alloputida, recipient cells typically had 2–6 sul1 copies with up to 14 per cell, and river biofilm samples enabled host–ARG tracking in situ.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.