The abstract is read at the publisher; the summary is JClub's.
Eleven weeks after fractionated whole-brain irradiation (5 x 4 Gy) in mice, neurons in both hippocampus and prefrontal cortex show persistent, cell-type-specific transcriptional and DNA methylation alterations.
- Why it matters: Fractionated whole-brain irradiation is essential for brain tumors, yet long-term survivors often develop cognitive decline with an unclear molecular basis.
- What they did: Male and female mice received whole-brain irradiation (5 x 4 Gy) or anesthesia alone, then at 11 weeks used single-nucleus RNA sequencing and whole-genome bisulfite sequencing on pooled hippocampal (CA1-3) and prefrontal tissue.
- The result: Both regions had reduced ribosomal-protein expression and adhesion changes, with region-specific respiratory-chain and synaptic gene patterns; differentially methylated regions were promoter-enriched with hypermethylation predominating in three of four sex-by-region contrasts, and methylation and expression changes overlapped in synaptic and signaling programs via largely different genes.
The findingWhy it mattersWhat they didThe result
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