The abstract is read at the publisher; the summary is JClub's.
Day 1 conditioned media from DOX-treated primary rat cardiac fibroblasts shows 35 proteins significantly linked to treatment, including collagen-centered and GAPDH/HSPA8/HSPA9 networks.
- Why it matters: Early secretory changes that occur before established senescence after doxorubicin exposure are not well characterized, despite links between fibroblast senescence, SASP, and cardiotoxic fibrosis.
- What they did: Primary cardiac fibroblasts from healthy rat hearts were exposed to DOX at 0–3 M for 24 hours; Day 1 media underwent untargeted proteomics with FDR correction, while viability, proliferation, and senescence-associated β-galactosidase were measured at Days 1 and 7.
- The result: Acute DOX lowered viability and proliferation at Day 1 without a significant β-galactosidase increase, while by Day 7 it showed delayed senescence; the Day 1 proteome implicates ECM regulation and mitochondrial stress/proteostasis pathways.
The findingWhy it mattersWhat they didThe result
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