The abstract is read at the publisher; the summary is JClub's.
Culture of human cardiac fibroblasts is linked to extensive remodeling of the cell surface proteome, with the largest surface protein abundance shifts between P0 and P1.
- Why it matters: Reliable CF cell-surface markers and targets are hard to validate because most studies rely on cultured cells, yet culture-associated surface changes are not well mapped.
- What they did: Using mass spectrometry-based surfaceome profiling across five sequential substudies of human CF (failing vs non-failing, fresh vs cryopreserved, lab vs commercial), they tracked passage-resolved trajectories and compared with whole-cell proteomics.
- The result: They built CFxplorer, including a Human CF Surfaceome Atlas of 1,577 N-glycoproteins across 234 CF samples, enabling prioritized marker/target validation and showing culture history should be verified.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.