The abstract is read at the publisher; the summary is JClub's.
scTPPM enables simultaneous single-cell profiling in mouse oocytes, identifying and quantifying 27,668 genes, 4,847 proteins, 1,508 PTM sites, and 680 metabolites per cell.
- Why it matters: Co-measuring nucleic acids, proteins, metabolites, and PTMs in the same individual cells remains difficult, limiting understanding of coordinated regulation during dynamic processes like oocyte meiosis.
- What they did: The authors built scTPPM to co-profile transcriptomes, proteomes, eight PTM types, and metabolomes, then applied it to single mouse oocytes to map pathway-level interactions.
- The result: The approach reveals dynamic crosstalk across entire metabolic pathways during oocyte maturation, linking metabolic enzyme expression and regulation to metabolite-mediated PTM changes.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.