The abstract is read at the publisher; the summary is JClub's.
Male-specific genome sequences in Nishikigoi (Cyprinus carpio) were identified and PCR markers were developed, mapping to chromosome 25B near the male-determining gene Pangu.
- Why it matters: Early, non-invasive sex identification is needed for commercial Nishikigoi breeding because mature females have higher market value, yet sex-chromosome markers and field diagnostics are limited.
- What they did: Using sequence-based bulked segregant analysis with whole-genome shotgun sequencing of female and male pools from Kohaku, the study built male-specific candidate contigs and PCR primer pairs, then validated them across multiple Nishikigoi varieties and wild common carp.
- The result: The primary primer pairs showed high diagnostic accuracy and, with non-invasive epidermal mucus DNA extraction plus LAMP and C-PAS, enabled a rapid on-site workflow for sex-linked detection.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.