The abstract is read at the publisher; the summary is JClub's.
Single nucleus RNA sequencing shows PUC bladder tumors have increased tumor–immune–stroma signaling involving CDH1-low urothelial cells, immunosuppressive lipid-associated macrophages, and SFRP2+/CTHRC1+ fibroblasts versus CUC.
- Why it matters: The tumor microenvironment and cell-to-cell crosstalk in primary PUC remain unclear, limiting understanding of how these rewired interactions may affect immunotherapy response.
- What they did: The team profiled PUC and CUC tumors with single nucleus RNA sequencing, and added Xenium spatial transcriptomics on a mixed CUC/PUC tumor to map where key cell types co-occur.
- The result: Spatial data linked PUC histology to higher macrophage infiltration and colocalization of PUC tumor cells, immunosuppressive macrophages, and SFRP2+ fibroblasts, suggesting PUC-specific therapeutic vulnerabilities.
The findingWhy it mattersWhat they didThe result
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