The abstract is read at the publisher; the summary is JClub's.
Methylene blue suppresses G3BP1-associated stress granule accumulation in multiple PDAC cell lines under arsenite, nutrient deprivation, and heat shock, without lowering major SG protein levels.
- Why it matters: Stress granules and their G3BP1/2 regulators are linked to pancreatic ductal adenocarcinoma biology, but small-molecule tools to modulate G3BP1 condensation dynamics are limited.
- What they did: The team screened FDA-approved drugs using structure-based methods, then tested methylene blue with cellular and biochemical assays, including reconstituted G3BP1 condensation ± RNA, docking/MD, and G3BP1 NTF2L-domain mutant analysis.
- The result: In Kras-driven KC mice, chronic oral methylene blue was associated with fewer pancreatic G3BP1-positive puncta and reduced preneoplastic lesion burden, epithelial proliferation, and stromal remodeling.
The findingWhy it mattersWhat they didThe result
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