Co-aggregation of pre-conditioned cell populations drives controlled lineage bias and formation of gut tube-like structures in human stem cell-based embryo models

Co-aggregating differentially pre-conditioned human pluripotent stem cell-derived cell pools in human stem cell-based embryo models yields consistent gut tube-like morphotypes with mesodermal lineages.

• Why it matters: Human stem cell-based embryo models often show variable growth and morphogenesis and incomplete lineage representation, limiting reliable study of post-implantation development.
• What they did: The authors built a modular aggregation method using co-aggregation of defined, pre-conditioned cell population pools and assessed morphogenesis outcomes in hSCBEMs.
• The result: The co-aggregation strategy improves reproducibility and developmental fidelity and enables supervised morphogenesis that robustly self-organizes gut tube-like structures with mesodermal lineages.

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