The abstract is read at the publisher; the summary is JClub's.
Constitutive SOX7 and ERG expression converts human pluripotent stem cell–derived epithelial barrier-forming cells into brain endothelial cells with a forebrain-biased regional program stronger than ETS- or umbilical-vein controls.
- Why it matters: Existing BBB models often select for barrier or transporter traits without achieving authentic, regionally specified brain endothelium identity, limiting physiological relevance.
- What they did: The authors reprogrammed iBFCs by constitutively expressing SOX7 and ERG, then benchmarked SE rEC transcriptome and proteome against a human adult-brain endothelial atlas and tested BBB maturation in neurovascular co-culture and perfused microfluidic devices.
- The result: In 2D neurovascular-unit and perfused microfluidic models, SE rECs with astrocytes and pericytes induced mature BBB features and reduced paracellular permeability, enabling a lineage-faithful, organotypically informed human BBB platform.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.