The abstract is read at the publisher; the summary is JClub's.
A single-residue switch in the aromatic prenyltransferase AtaPT from Aspergillus terreus governs both chemo- and regioselectivity, enabling selective mono-geranylation of cannabigerol.
- Why it matters: Geranylation needs both high efficiency and precise product control, but PTs often face a trade-off between broad substrate scope and low selectivity.
- What they did: The authors rationally tuned the AtaPT switch, used structural analysis to link steric and electrostatic effects to activity/selectivity, and transplanted the site into three homologous PTs.
- The result: The tuned system selectively geranylated 36 aromatic substrates to yield 41 products, and with geranyl donor regeneration supported preparative synthesis at titers up to 324 mg/L.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.