The abstract is read at the publisher; the summary is JClub's.
About 25% of patient-derived xenograft (PDX) genomic sequencing runs show substantial enrichment of human–murine leukemia virus chimeric DNA, with ~90% junctions bearing canonical MLV integration hallmarks.
- Why it matters: Humanized mouse models may be compromised by mouse-origin infections, yet susceptibility to murine pathogens is rarely evaluated. This work addresses how often murine leukemia virus integration occurs in the human genome within xenografts.
- What they did: The authors screened 1,619 publicly available sequencing runs from PDX models for murine leukemia virus integration signals, then analyzed human–MLV junctions for canonical boundaries and 4-bp target site duplications. They also confirmed a complete ecotropic MLV integration in the human genome using HiFi and Sanger sequencing.
- The result: They found many PDX models from Bxv1- mouse strains, including NSG, contaminated by ecotropic MLV (E-MLV), implying mouse-to-human retroviral integration is more common and less restrictive than previously appreciated.
The findingWhy it mattersWhat they didThe result
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