The abstract is read at the publisher; the summary is JClub's.
iPSC-derived cardiomyocytes show adult-like thick-filament protein stoichiometry, with myosin heavy-to-light chain ratios approaching the expected 2:4 and myosin-binding protein levels matching adult regions.
- Why it matters: It is unclear whether immature sarcomeric isoform expression in iPSC-CMs also reflects incorrect protein dosage, limiting their use for studying sarcomeric disease mechanisms.
- What they did: The authors integrated two quantitative proteomic datasets from human iPSC-CMs with published fetal and region-resolved adult human heart proteomes, comparing sarcomeric protein abundance and ratios.
- The result: Despite clustering with fetal heart and retaining fetal troponin I expression, thick-filament protein proportions closely matched adult myocardium, supporting iPSC-CMs for MYBPC3-associated hypertrophic cardiomyopathy linked to altered protein dosage.
The findingWhy it mattersWhat they didThe result
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