The abstract is read at the publisher; the summary is JClub's.
In Ugandan children with sickle cell anaemia receiving monthly sulfadoxine-pyrimethamine, quintuple Pfdhfr IRN/Pfdhps GE haplotypes dominated (85.7–100%), with emerging sextuple variants.
- Why it matters: Antifolate resistance is widespread in East Africa, yet monthly SP remains standard malaria chemoprevention; resistance markers and infection complexity in children with SCA were unclear.
- What they did: A molecular sub-study nested in the CHEMCHA trial genotyped Pfdhfr (N51I, C59R, S108N, I164L) and Pfdhps (A437G, K540E, A581G, A613S, A613T) from microscopy-confirmed clinical malaria samples using amplicon sequencing, and estimated COI with MOIRE.
- The result: COI was estimated for 67 samples and most infections were monoclonal (51/67, 76.1%); Pfdhps A581G rose from 0/44 at baseline to endline in Jinja and Kitgum, including sextuple haplotypes with Pfdhfr I164L and Pfdhps A581G.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.