Chemical crosslinking enables profiling of endogenous cell surface galectin-3 interactions

xGL-APMS identified 125 N-glycan branching-dependent cell-surface galectin-3 interactors on Jurkat cells and enabled comparison of low- versus high-dose galectin-3 interactomes.

• Why it matters: Mapping endogenous galectin-3 multivalent, low-affinity glycan interactions is technically difficult, limiting faithful profiling of its cell-surface interactome.
• What they did: The proteome-scale method captures native galectin-3 interactions at the cell surface without exogenous galectin-3, and it was validated with six orthogonal approaches.
• The result: Immediate crosslinking of endogenous galectin-3 on primary mouse thymocytes yielded 69 associated proteins, supporting xGL-APMS as a framework for measuring endogenous surface galectin-3 partners.

AI summary of the abstract · may contain errors · not medical advice · read the paper.