Evaluation of immunotoxic effects and metabolism of enniatins and beauvericin using in vitro models of the human intestinal epithelium

Enniatins (ENNB, ENNB1, ENNA, ENNA1) and beauvericin show limited acute toxicity in human intestinal models at 1–10 microM, while ENNA/ENNA1 strongly stimulate IL-6.

• Why it matters: The gastrointestinal tract is a primary exposure site from cereal foods, but the intestinal immunotoxicity and handling of these mycotoxins at low, dietary-relevant levels are not well defined.
• What they did: Using EpiIntestinal (TM) tissues and a Caco-2/TH29-MTX-E12 co-culture, the study tested cytotoxicity and barrier integrity, measured IL-6/IL-8 secretion, and quantified apical-to-basolateral transport and metabolite formation.
• The result: No cytotoxicity or barrier impairment occurred from 1 to 10 microM; ENNA and ENNA1 triggered the strongest IL-6 and IL-8 responses, while ENNB and ENNB1 crossed at ~32% and ~25% within 4 h and generated metabolites in tissues.

AI summary of the abstract · may contain errors · not medical advice · read the paper.