bioRxivJClub
Functional genomics reveals mediators of beta cell survival in ER stress and type 2 diabetes risk
bioRxiv · · Preprint · Open access
Okino, Zhu + 12 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
A CRISPR loss-of-function screen in EndoC-bH1 beta cells identified 167 pro-survival and 47 pro-death genes that mediate survival under ER stress.
- Why it matters: ER stress in pancreatic beta cells is linked to impaired function and type 2 diabetes, but the specific mediators connecting stress responses to T2D risk remain unclear.
- What they did: The study combined genome-wide perturbation screens with genomic profiling, built beta cell gene regulatory networks, and tested survival under ER stress using CRISPR in EndoC-bH1 cells.
- The result: Pro-survival genes showed limited genomic change yet were independently enriched for T2D risk variants, including DTNB, which was validated to protect against beta cell death during stress.
The findingWhy it mattersWhat they didThe result
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- Open access
- 1 cites