Insulin stimulation induces dynamic interaction between 14-3-3 and the C-terminal tail domain of Kinesin-2 subunit KIF3B in vitro

Insulin stimulation in HEK293T cells significantly enhances the interaction between 14-3-3ε and the kinesin-2 subunit KIF3B, with a dynamic, compartment-specific binding site.

• Why it matters: Kinesin-2 cargo specificity and regulation are not well understood, particularly how adaptor engagement is controlled in response to signaling cues.
• What they did: In HEK293T cells, splitAPEX2 proximity labelling and fluorescence lifetime imaging microscopy (FLIM) were used to quantify 14-3-3ε–KIF3B proximity and track interaction dynamics after insulin.
• The result: Time-resolved FLIM indicates spatial and temporal control, and mapping implicates two conserved serine residues in the C-terminal tail of KIF3B, supporting a conditional, reversible motora–adaptor association.

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