Multiplexed Targeted iprm-PASEF Assays for MALDI Mass Spectrometry Imaging of Lipids, NAD+ 2 , and Related Metabolites in CD38 Knockout Mice

The iprm-PASEF MALDI-MSI workflow enables confident MS2-based identification and relative quantification of NAD+ and lipid isomers in CD38-/- mouse liver, including a more than 2-fold NAD+ increase.

• Why it matters: In situ MALDI-MSI identification and accurate quantification of metabolites like NAD+ and lipid isomers are difficult, and CD38-/- biology is linked to age-related NAD+ decline.
• What they did: Liver tissue from wild-type and CD38-/- mice was analyzed using targeted iprm-PASEF with TIMS and MALDI, resolving phosphatidylinositols at m/z 857.52 and quantifying NAD+ via characteristic fragment ions.
• The result: Male and female CD38-/- livers (n = 3 per genotype and sex) showed more than 2-fold increased NAD+ and decreased ADPR, with consistent fold changes across fragment ions and biological replicates.

AI summary of the abstract · may contain errors · not medical advice · read the paper.