The abstract is read at the publisher; the summary is JClub's.
RhsP2, an RNA-targeting ADP-ribosyltransferase toxin in bacteria, targets mRNA coding sequences and blocks efficient translation while broadly stabilizing the modified transcripts.
- Why it matters: It was unclear whether RhsP2 has physiological mRNA substrates and what happens to transcript stability and protein output after toxin-mediated RNA modification.
- What they did: The study used eCLIP-seq to map RhsP2 binding to UAAG-enriched motifs, biochemical assays to show preferred ribose 2'-OH ADP-ribosylation, and monosome/disome-seq plus in vitro translation to test ribosome stalling and elongation blockade.
- The result: Modified mRNAs accumulate broadly stabilized despite impaired protein synthesis, and ADP-ribosylation is sufficient to block translation elongation, indicating toxin-mediated translational sabotage via chemically modified transcript buildup.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.