Nucleic Acids ResJClub
IsoAPA: a multi-species long-read RNA-seq atlas of isoform-resolved alternative polyadenylation.
Nucleic Acids Research · · Journal Article
Zhang, Yau + 1 more
Abstract ↗AI summary
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IsoAPA provides over 2.3 million transcript-resolved alternative polyadenylation (APA) records across 99 species, preserving native isoform context for each PAS.
- Why it matters: Gene-level catalogs of polyadenylation sites miss which transcript isoforms actually use each site, limiting isoform-resolved understanding of transcript-end regulation.
- What they did: The database curates PacBio and Oxford Nanopore long-read RNA-seq to assign PASs to transcript models, yielding 2.3 million terminal-site records from 816 sample groups.
- The result: In human data, the union across 286 sample groups shows a mean of 2.52 distinct retained PASs per observed transcript, enabling isoform-resolved PAS diversity and context-dependent dominant-site usage.
The findingWhy it mattersWhat they didThe result
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