Case Study of a Cross-Platform Evaluation of an Optimized Multicolor Flow Cytometry Immunophenotyping Assay for Human Peripheral Whole Blood.

Absolute concentrations of cell populations differed when an optimized multicolor flow cytometry immunophenotyping assay was transferred from the Gallios to the DxFlex, despite successful measurement on DxFlex.

• Why it matters: Clinical immune monitoring assays must be re-established on new flow cytometers to maintain stable longitudinal measurements, but device changes can introduce comparability problems.
• What they did: The assay was optimized and transferred from a Gallios to a DxFlex (both Beckman Coulter) using comparable lasers and filters, then parallel blood measurements were analyzed for population concentration deviations.
• The result: Calculated absolute values cannot be compared across these two devices, implying ongoing trials should not switch this IPT assay without parallel comparative measurements to preserve detection and result comparability.

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