Plant Physiol BiochemJClub
Sup4h5-L19 activation-tagging line partially restores root hair growth in the p4h5 mutant of Arabidopsis thaliana.
Plant Physiology and Biochemistry · · Journal Article · Open access
MÁ, Salter + more
Abstract ↗AI summary
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Sup4h5-L19/p4h5 partially restores root hair growth in the Arabidopsis thaliana p4h5 mutant, with P4H5 transcript levels reaching 39–59% of Wt Col-0 at 10°C.
- Why it matters: Root hair elongation depends on P4H5-mediated proline hydroxylation, yet the p4h5 mutant shows arrested elongation and shortened hairs, leaving downstream low-temperature components unclear.
- What they did: The authors mapped the T-DNA insertion in Sup4h5-L19/p4h5 to the start of the sixth exon of AT3G17750 and used RNA-seq to compare p4h5-L19 versus p4h5 in Wt Col-0, focusing on 10°C.
- The result: Activation-tagging L19 altered 18 genes upward and 79 downward, including partial P4H5 transcript restoration and root-hair-expressed TIP genes whose mutants showed reduced low-temperature root hair response.
The findingWhy it mattersWhat they didThe result
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