Identification and functional characterization of pineapple AcGPX5 in oxidative stress response.

AcGPX5 from pineapple (Ananas comosus) shows thioredoxin-dependent glutathione peroxidase activity and is linked to essential oxidative stress resistance in transgenic plants.

• Why it matters: Terminal homozygosity regions formed by mitotic recombination are understudied, and their functional contribution to fruit ripening and stress biology is unclear.
• What they did: The study analyzed genes in these regions, clustered ripening-stage transcriptomic profiles into five expression groups, then sequence- and localization-characterized AcGPX5 and tested recombinant enzyme activity plus transgenic lines.
• The result: Enzyme assays confirmed thioredoxin-dependent glutathione peroxidase activity, and transgenic experiments indicated AcGPX5 is essential for oxidative stress resistance, supporting its role as a ripening-related candidate.

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