Functional involvement of AMPK in Lumpy skin disease virus replication and associated cellular stress responses.

LSDV infection in MAC-T cells activates AMPK, with Thr172 phosphorylation increasing, and AMPK activity is linked to enhanced viral replication.

• Why it matters: The study addresses how LSDV-driven energy, oxidative, mitochondrial, and ER stress signals are connected to viral replication, which remains unclear.
• What they did: MAC-T cells were infected with LSDV and assessed by transcriptomics, transmission electron microscopy, ATP/AMP and lactate metabolism, mitochondrial calcium, reactive oxygen species, and ER stress signaling; AMPK was tested using inhibition and downstream autophagy readouts.
• The result: AMPK inhibition impaired LSDV replication, and AMPK activation promoted autophagosome formation, implying AMPK supports replication via autophagy in this in vitro model.

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