Subtractive genetic circuits improve organoid development.

Subtractive synthetic gene circuits in an iPSC line delete less mature cells in developing liver organoids and embryoids, yielding more mature phenotypes.

• Why it matters: Removing unwanted, less mature cell populations is a key challenge for improving organoid development and achieving desired tissue maturation.
• What they did: Taheri, Xiang, et al. engineered subtractive genetic circuits in an iPSC line and applied them during development of liver organoids and embryoids to target less mature cells.
• The result: Deleting these less mature cells is linked to more mature phenotypes in both liver organoids and embryoids, supporting a strategy to tune organoid maturation.

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