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PerCap isolates native peroxisomes to reveal peroxisomal proteins and regulators.
Nature Chemical Biology · · Journal Article
Chen, Dang + more
Abstract ↗AI summary
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PerCap affinity purification isolates intact native peroxisomes from human cells and mouse tissues, enabling proteomic identification of peroxisomal proteins and regulators.
- Why it matters: Peroxisomes vary widely in function, but studying their roles and regulation requires isolating native organelles for proteomics.
- What they did: The N-terminal region of ATF6α binds directly to ABCD1 and ABCD3 in vitro, and PerCap uses this interaction to purify peroxisomes, then applies proteomic analysis and functional tests.
- The result: AKAP1, CPT1A, and CYB5R1 localize to peroxisomes, AKAP1 loss alters peroxisome morphology, and Torin1-induced pexophagy proteome dynamics implicate UBE2V1.
The findingWhy it mattersWhat they didThe result
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