Paper Strip-Based Sensor for Detection of Antimicrobial Resistant Listeria monocytogenes in Milk.

A paper strip assay detected Listeria monocytogenes in spiked sterilized milk within 9 h after 24 h enrichment at 1 CFU/25 mL, with color-based confirmation.

• Why it matters: Rapid, field-deployable detection in milk is needed, alongside targeted screening for resistance to key antibiotics, to speed decisions without full susceptibility testing.
• What they did: The team optimized a Listeria Selective Enrichment Medium (LSEM) to improve recovery and reduce competing interference, then used β-D-glucosidase and enzyme-substrate reactions on paper strips.
• The result: Resistant strains developed color with Penicillin G or Ampicillin while susceptible strains stayed colorless, and testing 70 milk samples found one raw milk positive, verified by ISO 11290-1:2017 and disc diffusion.

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