Nat MethodsJClub
Aptamer-mediated multivalent endogenous ADAR1 recruitment for highly efficient RNA editing.
Nature Methods · · Journal Article
Zhu, Zhou + more
Abstract ↗AI summary
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MARRS recruits endogenous ADAR1 via multivalent aptamers to achieve near-complete adenosine-to-inosine RNA editing, reaching up to 100% in vitro and 84% in mouse models.
- Why it matters: RNA editing therapies are promising but limited by low editing efficiency, which constrains practical therapeutic use.
- What they did: The authors engineered multivalent RNA aptamers targeting the Zα domain of ADAR1 p150 and embedded them in a circular RNA scaffold to recruit endogenous ADAR1 in cultured cells and mouse models.
- The result: MARRS enables highly efficient, specific editing across multiple transcript targets with minimal off-target effects, without exogenous enzymes, supporting a programmable therapeutic platform.
The findingWhy it mattersWhat they didThe result