bioRxivJClub
m6A regulates ADAR1 mediated RNA editing during macrophage activation
bioRxiv · · Preprint · Open access
Di Giorgio, Griesche + 7 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
In LPS/IFN-γ–stimulated RAW 264.7 macrophages, about 60% of expressed genes are m6A-modified and ~12% harbor A-to-I editing, with ~10% carrying both marks.
- Why it matters: The interplay between m6A and ADAR1-mediated A-to-I editing is unresolved because earlier methods used incompatible ways to assign each modification.
- What they did: RAW 264.7 cells were made deficient for METTL3 or ADAR1, and Nanopore direct RNA sequencing (with Illumina validation when appropriate) was used to assess co-occurrence of m6A and A-to-I on individual transcripts.
- The result: This enables linking modification co-occurrence to macrophage activation outputs in a mechanistic system, using METTL3- or ADAR1-deficient cells to test how each mark relates to the other.
The findingWhy it mattersWhat they didThe result
- Open access