rustybam: a composable toolkit for alignment analysis and visualization with SafFire.

rustybam is a Rust-based command-line toolkit for CIGAR-aware manipulation of PAF and BAM alignments, paired with SafFire for interactive genome comparison visualization.

• Why it matters: Existing alignment workflows often need consistent, CIGAR-aware operations and interactive comparison views, but integrated tooling for these tasks is limited.
• What they did: rustybam provides composable subcommands for overlap resolution, coordinate liftover, alignment splitting at structural variants, scaffolding, and identity statistics. SafFire adds browser-based interactive genome comparison visualization with annotation overlays.
• The result: The open-source tools (MIT license) are available via Bioconda, crates.io, GitHub, and archived Zenodo releases, enabling reproducible alignment analysis and visualization.