Generation of autopsy-derived human iPSC lines from 24 sporadic Alzheimer's disease and control donors characterized for polygenic AD risk.

Twenty-four autopsy-derived human iPSC lines were generated from Seattle, WA sporadic Alzheimer’s disease donors and controls, spanning a range of AD neuropathology and comorbid aging-related disorders.

• Why it matters: The work addresses the need for cellular models that preserve donor-specific clinical, genetic, and neuropathologic context to study genetic contributions to sporadic AD and support drug screening.
• What they did: Leptomeningeal tissue collected at brain removal was used to derive primary fibroblasts, which were reprogrammed into hiPSC lines; each donor has extensive clinical, genetic, and neuropathologic data including polygenic Alzheimer’s disease risk genotyping through the endolysosomal pathway.
• The result: These characterized hiPSC lines enable studies of cellular mechanisms linked to AD pathogenesis and provide a donor-matched screening tool for drug development.